PHYSICAL AND CHEMICAL MUTAGENESIS IN CATHARANTHUS ROSEUS PLANT USING SCOT MARKERS

The current study comprising molecular identification of the mutagenic effects gamma rays and sodium azide on Catharanthus roseus using scot markers took place at Department Biology, University Kufa, Iraq. seeds two cultivars, i.e., ‘victory carmine’ a local variety (pink variety) L (carmine var.) received irradiation with doses (50 Gray 75 Gray). Afterward, these got soaked in four concentrations (0.2%, 0.4%, 0.6%, 0.8%) to induce genetic variation cultivar. non-treated served as control treatments for comparison treated ones. Accomplishing was an average 18 Gy/h Cobalt-60. treatments’ evaluation were through 10 start codon targeted (SCoT) DNA markers. higher number distinctive fingerprints three produced by primers SCoT-26, while primer SCoT-60 provided distinct fingerprint only one treatment. However, SCoT-40 failed recognize any treatment fingerprint. highest size (2488 bp) came from SCoT-54, whereas lower (143 resulted SCoT-12. Primer SCoT-33 value chief unique bands, reaching 33 18, respectively. amplified bands 77 SCoT-54. monomorphic SCoT-12 gave polymorphic polymorphism, discriminatory (15, 68.18, 20.83, respectively), SCoT-9 efficiency.